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Soares_fetal_liver_spleen_1NFLS_S1

Identifiers
BioSample: SAMN00155062; EST: LIBEST_000452
Organism
Homo sapiens (human)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Primates; Haplorrhini; Simiiformes; Catarrhini; Hominoidea; Hominidae; Homininae; Homo
Attributes
sexmale
development stage20 week-post conception fetus
lab hostDH10B (ampicillin resistant)
tissueLiver and Spleen
vectorpT7T3D (Pharmacia) with a modified polylinker
v_typephagemid
re_1Pac I
re_2Eco RI
Description

This is a subtracted version of the original Soares fetal liver spleen 1NFLS library. 1st strand cDNA was primed with a Pac I - oligo(dT) primer [5' AACTGGAAGAATTAATTAAAGATCTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Pac I and cloned into the Pac I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization. Library constructed by Bento Soares and M.Fatima Bonaldo.

Submission
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Inge Arlart; 1996-04-04
Accession:
SAMN00155062
ID:
155062

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