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Status |
Public on Feb 15, 2010 |
Title |
HuR differentially regulates unique subsets of mRNAs in estrogen receptor negative and positive breast cancer |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Analysis of RNA immunoprecipitation of HuR, a RNA binding protein (RBP), in breast cancer cell lines. This approach, utilizing RNA immunoprecipitation hybridized to microarray (RIP-Chip), provides global identification of putative endogenous mRNA targets of different RBPs. HuR is an RBP that binds to the AU-rich (ARE) regions of labile mRNAs, such as proto-oncogenes, facilitating their translation into protein. HuR has been shown to play a role in cancer progression and elevated levels of cytoplasmic HuR directly correlate with increased invasiveness and poor prognosis for many cancers, including those of the breast. We used HuR RIP-Chip as a comprehensive and systematic method to survey breast cancer target genes in both MCF-7 (estrogen receptor positive, ER+) and MDA-MB-231 (estrogen receptor negative, ER-) breast cancer cell lines. We identified unique subsets of HuR associated mRNAs found individually or in both cell types. Two novel HuR targets, CD-9 and CALM-2, were identified and validated by quantitative RT-PCR and biotin pulldown analysis. Our findings reveal that the differential regulation of these two cancer-related genes by HuR was contingent upon the cellular environment.
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Overall design |
RNA immunoprecipitation of the HuR RNA binding protein by 3A2 antibody and IgG (control) from two human breast cancer cell lines, MCF-7 and MDA-MB-231 .
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Contributor(s) |
Atasoy U, Davis JW, Calaluce R, Grubin M, Magee J, Kuwano Y, Gorospe M |
Citation(s) |
20370918 |
Submission date |
Aug 26, 2009 |
Last update date |
Jan 08, 2019 |
Contact name |
J Wade Davis |
Organization name |
AbbVie
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Street address |
1 N Waukegan Rd
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City |
North Chicago |
State/province |
IL |
ZIP/Postal code |
60048 |
Country |
USA |
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Platforms (1) |
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Samples (12)
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Relations |
BioProject |
PRJNA120039 |
Supplementary file |
Size |
Download |
File type/resource |
GSE17820_non-normalized.txt.gz |
7.6 Mb |
(ftp)(http) |
TXT |
Processed data included within Sample table |
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