Abstract
We have transformed potato with Nt-inhh cDNA, encoding a putative vacuolar homolog of a tobacco cell wall invertase inhibitor, under the control of the CaMV 35S promoter. In transgenic tubers, cold-induced hexose accumulation was reduced by up to 75%, without any effect on potato tuber yield. Processing quality of tubers was greatly improved without changing starch quantity or quality, an important prerequisite for the biotechnological use of Nt-inhh for potato transformation.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Biotechnology
-
Cell Wall / enzymology
-
Cloning, Molecular
-
Cold Temperature
-
Enzyme Inhibitors*
-
Gene Expression Regulation, Enzymologic
-
Glycoside Hydrolases / antagonists & inhibitors*
-
Glycoside Hydrolases / metabolism
-
Hexoses / metabolism*
-
Molecular Sequence Data
-
Nicotiana / enzymology
-
Nicotiana / genetics*
-
Plant Proteins / chemistry
-
Plant Proteins / genetics*
-
Plant Proteins / metabolism
-
Plants, Toxic*
-
Solanum tuberosum / enzymology
-
Solanum tuberosum / genetics*
-
Transformation, Genetic
-
Vacuoles / enzymology
-
beta-Fructofuranosidase
Substances
-
Enzyme Inhibitors
-
Hexoses
-
INH protein, Nicotiana tabacum
-
Plant Proteins
-
Glycoside Hydrolases
-
beta-Fructofuranosidase