Abstract
A multiplex PCR was designed to detect the eae gene and simultaneously identify specific alleles in pathogenic Escherichia coli. The method was tested on 87 strains representing the diarrheagenic E. coli clones. The results show that the PCR assay accurately detects eae and resolves alleles encoding the alpha, beta, and gamma intimin variants.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adhesins, Bacterial*
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Alleles
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Animals
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Bacterial Outer Membrane Proteins / genetics*
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Carrier Proteins*
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Escherichia coli / genetics*
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Escherichia coli / isolation & purification
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Escherichia coli / pathogenicity
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Escherichia coli Infections / microbiology*
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Escherichia coli Proteins*
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Humans
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Polymerase Chain Reaction
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Virulence / genetics
Substances
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Adhesins, Bacterial
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Bacterial Outer Membrane Proteins
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Carrier Proteins
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Escherichia coli Proteins
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eaeA protein, E coli