Abstract
Mouse NK cells express at least seven inhibitory Ly49 receptors. Here we employ a semiquantitative cell-cell adhesion assay as well as class I/peptide tetramers to provide a comprehensive analysis of specificities of Ly49 receptors for class I MHC molecules in eight MHC haplotypes. Different Ly49 receptors exhibited diverse binding properties. The degree of class I binding was related to the extent of functional inhibition. The tetramer studies demonstrated that neither glycosylation nor coreceptors were necessary for class I binding to Ly49 receptors and uncovered peptide-specific recognition by a Ly49 receptor. The results provide a foundation for interpreting and integrating many existing functional studies as well as for designing tests of NK cell development and self-tolerance.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Alleles
-
Animals
-
Antigens, Ly*
-
Carrier Proteins / metabolism
-
Cell Adhesion / genetics
-
Cell Adhesion / immunology
-
H-2 Antigens / metabolism
-
Histocompatibility Antigens Class I / genetics
-
Histocompatibility Antigens Class I / metabolism*
-
Immunosuppressive Agents / metabolism*
-
Immunosuppressive Agents / pharmacology
-
Killer Cells, Natural / immunology*
-
Killer Cells, Natural / metabolism*
-
Lectins, C-Type
-
Lymphocyte Culture Test, Mixed
-
Membrane Glycoproteins / genetics
-
Membrane Glycoproteins / metabolism*
-
Membrane Glycoproteins / pharmacology
-
Membrane Proteins / metabolism
-
Mice
-
Mice, Congenic
-
Mice, Inbred A
-
Mice, Inbred C57BL
-
Mice, Inbred CBA
-
Mice, Transgenic
-
Peptides / metabolism
-
Protein Binding / genetics
-
Protein Binding / immunology
-
Receptors, Immunologic / genetics
-
Receptors, Immunologic / metabolism*
-
Receptors, Immunologic / physiology
-
Receptors, NK Cell Lectin-Like
-
Solubility
Substances
-
Antigens, Ly
-
Carrier Proteins
-
H-2 Antigens
-
H-2K(K) antigen
-
Histocompatibility Antigens Class I
-
Immunosuppressive Agents
-
Lectins, C-Type
-
Membrane Glycoproteins
-
Membrane Proteins
-
Peptides
-
Receptors, Immunologic
-
Receptors, NK Cell Lectin-Like