Abstract
A cDNA library was prepared from Artemisia annua, and a 129-bp fragment was amplified from this library using primers corresponding to sequences conserved in known dicot sesquiterpene synthases. A 1641-bp open reading frame that encoded a predicted protein 35-38% identical to dicot sesquiterpene synthases was cloned using this fragment as a hybridization probe. The gene product expressed in Escherichia coli cyclized farnesyl diphosphate to a 96:4 mixture of (-)8-epicedrol and cedrol. Neither cedrol epimer was detected by GC-MS in an A. annua extract prepared from the same specimen as the cDNA.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Antimalarials / metabolism
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Artemisinins*
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Asteraceae / enzymology
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Asteraceae / genetics*
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Carbon-Carbon Lyases / genetics*
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Carbon-Carbon Lyases / metabolism
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Cloning, Molecular
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DNA, Complementary / genetics
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Escherichia coli / genetics
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Gene Library
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Genes, Plant
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Models, Chemical
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Molecular Sequence Data
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Nuclear Magnetic Resonance, Biomolecular
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Plants, Medicinal / enzymology
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Plants, Medicinal / genetics*
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Polycyclic Sesquiterpenes
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Polyisoprenyl Phosphates / metabolism*
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RNA, Plant / genetics
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Recombinant Proteins / metabolism
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Sequence Homology, Amino Acid
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Sesquiterpenes / metabolism
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Terpenes / chemical synthesis*
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Terpenes / chemistry
Substances
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Antimalarials
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Artemisinins
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DNA, Complementary
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Polycyclic Sesquiterpenes
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Polyisoprenyl Phosphates
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RNA, Plant
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Recombinant Proteins
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Sesquiterpenes
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Terpenes
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cedrol
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farnesyl pyrophosphate
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artemisinin
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Carbon-Carbon Lyases
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8-epicedrol synthase