Abstract
The contribution of the dosage of target enzyme P-450 14alpha-demethylase (14alphaDM) to fluconazole resistance in both Candida albicans and Saccharomyces cerevisiae remains unclear. Here, we show that overexpression of Saccharomyces P-450 14alphaDM in S. cerevisiae, under the control of the regulatable promoter GAL1, results in azole resistance.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antifungal Agents / pharmacology*
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Cytochrome P-450 Enzyme System / biosynthesis*
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Cytochrome P-450 Enzyme System / genetics
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DNA, Fungal / genetics
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DNA, Fungal / isolation & purification
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Drug Resistance, Microbial
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Fluconazole / pharmacology*
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Fungal Proteins / biosynthesis*
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Fungal Proteins / genetics
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Galactokinase / biosynthesis*
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Galactokinase / genetics
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Oxidoreductases / biosynthesis*
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Oxidoreductases / genetics
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Point Mutation / genetics
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Promoter Regions, Genetic / genetics*
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Saccharomyces cerevisiae / drug effects*
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Saccharomyces cerevisiae / genetics*
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Sterol 14-Demethylase
Substances
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Antifungal Agents
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DNA, Fungal
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Fungal Proteins
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Fluconazole
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Cytochrome P-450 Enzyme System
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Oxidoreductases
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Sterol 14-Demethylase
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Galactokinase