Reovirus messenger RNA contains a methylated, blocked 5'-terminal structure: m-7G(5')ppp(5')G-MpCp-

Proc Natl Acad Sci U S A. 1975 Jan;72(1):362-6. doi: 10.1073/pnas.72.1.362.

Abstract

Reovirus mRNA synthesized in vitro by the virus-associated RNA polymerase in the presence of S-adenosylmethionine contains blocked, methylated 5'-termini with the structure, m-7G(5')ppp(5')G-MpCp. The functional significance and possible mechanism of formation of this novel 5'-5' terminal nucleotide linkage are discussed.

MeSH terms

  • Animals
  • Base Sequence
  • Borohydrides / metabolism
  • Carbon Radioisotopes
  • Cell-Free System
  • DNA-Directed RNA Polymerases / metabolism
  • Enzyme Precursors / metabolism
  • Genotype
  • Guanosine Triphosphate / metabolism
  • Methylation
  • Mice
  • Phosphorus Radioisotopes
  • Protein Biosynthesis
  • Protein Precursors / metabolism
  • RNA, Messenger / biosynthesis*
  • RNA, Viral / biosynthesis*
  • Reoviridae / metabolism*
  • S-Adenosylmethionine / metabolism
  • Transcription, Genetic
  • Tritium
  • Viral Proteins / metabolism

Substances

  • Borohydrides
  • Carbon Radioisotopes
  • Enzyme Precursors
  • Phosphorus Radioisotopes
  • Protein Precursors
  • RNA, Messenger
  • RNA, Viral
  • Viral Proteins
  • Tritium
  • S-Adenosylmethionine
  • Guanosine Triphosphate
  • DNA-Directed RNA Polymerases