Abstract
Reovirus mRNA synthesized in vitro by the virus-associated RNA polymerase in the presence of S-adenosylmethionine contains blocked, methylated 5'-termini with the structure, m-7G(5')ppp(5')G-MpCp. The functional significance and possible mechanism of formation of this novel 5'-5' terminal nucleotide linkage are discussed.
MeSH terms
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Animals
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Base Sequence
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Borohydrides / metabolism
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Carbon Radioisotopes
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Cell-Free System
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DNA-Directed RNA Polymerases / metabolism
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Enzyme Precursors / metabolism
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Genotype
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Guanosine Triphosphate / metabolism
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Methylation
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Mice
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Phosphorus Radioisotopes
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Protein Biosynthesis
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Protein Precursors / metabolism
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RNA, Messenger / biosynthesis*
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RNA, Viral / biosynthesis*
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Reoviridae / metabolism*
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S-Adenosylmethionine / metabolism
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Transcription, Genetic
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Tritium
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Viral Proteins / metabolism
Substances
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Borohydrides
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Carbon Radioisotopes
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Enzyme Precursors
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Phosphorus Radioisotopes
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Protein Precursors
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RNA, Messenger
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RNA, Viral
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Viral Proteins
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Tritium
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S-Adenosylmethionine
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Guanosine Triphosphate
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DNA-Directed RNA Polymerases