Abstract
We cloned and sequenced a 2.7-kb fragment of chromosomal DNA from Clostridium perfringens containing the superoxide dismutase-encoding gene, sod. Previously, rubrerythrin from C. perfringens had been isolated and its gene (rbr) had been cloned (Y. Lehmann, L. Meile, and M. Teuber, J. Bacteriol. 178:7152-7158, 1996). Northern blot experiments revealed a length of approximately 800 bases for each transcript of rbr and sod of C. perfringens. Thus, rbr and sod each represent a monocistronic operon. Their transcription start points were located by primer extension analyses. sod transcription was shown to depend on the growth phase, and it reached a maximum during the transition from log phase to stationary phase. Neither sod nor rbr transcription was influenced by oxidative stress.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Base Sequence
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Cloning, Molecular
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Clostridium perfringens / enzymology
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Clostridium perfringens / genetics*
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Clostridium perfringens / growth & development
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Ferredoxins / chemistry
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Ferredoxins / genetics*
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Ferredoxins / metabolism
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Genes, Bacterial
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Hemerythrin
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Molecular Sequence Data
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Promoter Regions, Genetic
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Rubredoxins
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Sequence Analysis, DNA
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Superoxide Dismutase / chemistry
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Superoxide Dismutase / genetics*
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Superoxide Dismutase / metabolism
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Transcription, Genetic*
Substances
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Bacterial Proteins
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Ferredoxins
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Hemerythrin
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Rubredoxins
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rubrerythrins
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Superoxide Dismutase
Associated data
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GENBANK/X92844
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GENBANK/Y10531