Abstract
In vivo expression technology was used for testing Pseudomonas aeruginosa in the rat lung model of chronic infection and in a mouse model of systemic infection. Three of the eight ivi proteins found showed sequence identity to known virulence factors involved in iron acquisition via an open reading frame (called pvdI) implicated in pyoverdine biosynthesis, membrane biogenesis (FtsY), and adhesion (Hag2).
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Bacterial Adhesion / genetics
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Bacterial Proteins / biosynthesis
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Cell Membrane / genetics
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Cell Membrane / metabolism
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Colony Count, Microbial
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Gene Library
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Liver / microbiology
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Lung / microbiology
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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Oligopeptides*
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Open Reading Frames
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Pigments, Biological / biosynthesis
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Pigments, Biological / genetics
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Promoter Regions, Genetic
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Pseudomonas Infections / genetics*
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Pseudomonas aeruginosa / genetics*
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Rats
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Rats, Sprague-Dawley
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Receptors, Cytoplasmic and Nuclear / biosynthesis
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Recombinant Fusion Proteins / metabolism
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Virulence
Substances
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Bacterial Proteins
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FtsY protein, Bacteria
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Oligopeptides
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Pigments, Biological
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Receptors, Cytoplasmic and Nuclear
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Recombinant Fusion Proteins
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pyoverdin
Associated data
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GENBANK/AF214673
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GENBANK/AF214674
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GENBANK/AF214675
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GENBANK/AF214676
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GENBANK/AF214677
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GENBANK/AF214678
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GENBANK/AF214679