Abstract
Lactoferrin was hydrolysed with pepsin and the antimicrobial activity of the resulting hydrolysate (HLF) was studied in 1% peptone, 0.05% yeast extract, 1% glucose (PYG) medium and tryptic soy broth (TSB). HLF was effective against Listeria monocytogenes, enterohaemorrhagic Escherichia coli and Salmonella enteritidis in PYG, however, the highest studied concentration (1.6 mg ml-1) did not inhibit growth of any of these organisms in TSB. The addition of EDTA enhanced the activity of HLF in TSB, indicating that the decreased activity of HLF may have been due, in part, to excess cations in the medium.
MeSH terms
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Animals
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Bacteria / drug effects*
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Bacteria / growth & development
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Cattle
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Culture Media
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Edetic Acid / pharmacology*
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Escherichia coli / drug effects
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Escherichia coli / growth & development
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Food Microbiology*
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Lactoferrin / pharmacology*
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Listeria monocytogenes / drug effects
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Listeria monocytogenes / growth & development
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Microbial Sensitivity Tests
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Pepsin A / metabolism
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Salmonella enteritidis / drug effects
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Salmonella enteritidis / growth & development
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Soybean Proteins / metabolism
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Soybean Proteins / pharmacology
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Trypsin / metabolism
Substances
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Culture Media
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Soybean Proteins
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Edetic Acid
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Lactoferrin
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Trypsin
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Pepsin A