Abstract
Transcriptional activity in yeast strongly correlates with promoter occupancy by general factors such as TATA binding protein (TBP), TFIIA, and TFIIB, but not with occupancy by TBP-associated factors (TAFs). Thus, TBP exists in at least two transcriptionally active forms in vivo. The TAF-containing form corresponds to the TFIID complex, whereas the form lacking TAFs corresponds to TBP itself or to some other TBP complex. Heat shock treatment altered the relative utilization of these TBP forms, with TFIID being favored. Promoter-specific variations in the association of these distinct forms of TBP may explain why only some yeast genes require TFIID for transcriptional activity in vivo.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acetyltransferases / physiology
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DNA Polymerase II / metabolism
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Fungal Proteins / physiology
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Heat-Shock Proteins / physiology
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Histone Acetyltransferases
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Promoter Regions, Genetic
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae Proteins*
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TATA Box*
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TATA-Box Binding Protein
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Trans-Activators / metabolism
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Trans-Activators / physiology*
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Transcription Factor TFIIA
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Transcription Factor TFIIB
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Transcription Factor TFIID
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Transcription Factors / physiology
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Transcription Factors, TFII / physiology
Substances
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DNA-Binding Proteins
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Fungal Proteins
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Heat-Shock Proteins
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SAGA protein, Emericella nidulans
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Saccharomyces cerevisiae Proteins
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TATA-Box Binding Protein
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Trans-Activators
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Transcription Factor TFIIA
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Transcription Factor TFIIB
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Transcription Factor TFIID
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Transcription Factors
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Transcription Factors, TFII
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Acetyltransferases
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Histone Acetyltransferases
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DNA Polymerase II