Abstract
Bacterial isolates were obtained from pine (Pinus sylvestris L.) tissue cultures and identified as Methylobacterium extorquens and Pseudomonas synxantha. The existence of bacteria in pine buds was investigated by 16S rRNA in situ hybridization. Bacteria inhabited the buds of every tree examined, primarily colonizing the cells of scale primordia and resin ducts.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cycadopsida / microbiology*
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DNA, Bacterial / analysis
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DNA, Bacterial / genetics
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DNA, Ribosomal / analysis
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DNA, Ribosomal / genetics
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In Situ Hybridization
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Methylobacterium extorquens / classification
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Methylobacterium extorquens / genetics
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Methylobacterium extorquens / isolation & purification*
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Molecular Sequence Data
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Pinus sylvestris
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Pseudomonas / classification
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Pseudomonas / genetics
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Pseudomonas / isolation & purification*
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RNA, Ribosomal, 16S
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Sequence Analysis, DNA
Substances
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DNA, Bacterial
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DNA, Ribosomal
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RNA, Ribosomal, 16S
Associated data
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GENBANK/AF267911
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GENBANK/AF267912