Abstract
Some echoviruses (EV) that bind decay-accelerating factor (DAF) also bind cells of human and murine origins in a DAF-independent manner. Pretreatment of cells with heparinase 1 or heparin blocks the binding of radiolabeled virus to the cell surface, and heparin prevents infection of rhabdomyosarcoma cells by certain EV, including several low-passage clinical isolates of EV 6 and some EV that do not bind DAF. These studies suggest that heparan sulfate may be of in vivo relevance as an attachment molecule for EV.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3 Cells
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Animals
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CD55 Antigens / genetics
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CD55 Antigens / physiology
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CHO Cells
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Cell Membrane / metabolism
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Cricetinae
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Enterovirus B, Human / metabolism*
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Heparin / analogs & derivatives
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Heparin / metabolism
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Heparin Lyase / metabolism
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Heparitin Sulfate / metabolism*
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Humans
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Mice
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Receptors, Virus / metabolism*
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Type C Phospholipases / metabolism
Substances
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CD55 Antigens
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Receptors, Virus
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heparin, N-desulfated
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Heparin
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Heparitin Sulfate
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Type C Phospholipases
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Heparin Lyase