Abstract
The contribution of efflux pumps to multidrug resistance in 12 Pseudomonas aeruginosa isolates from various animal sources was assessed. Western immunoblot analyses demonstrated that all twelve isolates expressed significant levels of the MexAB OprM efflux system whereas two isolates simultaneously expressed the MexEF OprN or MexXY systems, respectively. One strain contained a single mutation in mexR, a regulator of mexAB-oprM expression, that did not adversely affect the MexR amino acid sequence, and three isolates contained the same, single base change in the mexA-mexR intergenic region. The MexXY-expressing strain contained two base substitutions in its mexZ regulatory gene which did not alter the MexR sequence.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Anti-Bacterial Agents / pharmacology
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Bacterial Outer Membrane Proteins / genetics
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Bacterial Outer Membrane Proteins / metabolism
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Bacterial Proteins*
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Base Sequence
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Carrier Proteins / genetics
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Carrier Proteins / metabolism
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Drug Resistance, Microbial / genetics*
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Drug Resistance, Multiple / genetics*
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Gene Expression Regulation, Bacterial
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Introns
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Membrane Transport Proteins*
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Pseudomonas Infections / microbiology
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Pseudomonas Infections / veterinary*
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Pseudomonas aeruginosa / drug effects*
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Pseudomonas aeruginosa / genetics*
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Pseudomonas aeruginosa / isolation & purification
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Repressor Proteins / chemistry
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Repressor Proteins / genetics*
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Repressor Proteins / metabolism
Substances
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Anti-Bacterial Agents
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Bacterial Outer Membrane Proteins
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Bacterial Proteins
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Carrier Proteins
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Membrane Transport Proteins
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MexA protein, Pseudomonas aeruginosa
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MexB protein, Pseudomonas aeruginosa
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MexR protein, Pseudomonas aeruginosa
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OprM protein, Pseudomonas aeruginosa
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OprN protein, Pseudomonas aeruginosa
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Repressor Proteins