Abstract
This study was performed to investigate whether colorectal cancers produce proliferative factors for venous endothelial cells, and whether the proliferative activity is related to basic FGF and VEGF and also to the clinicopathological findings. Surgically resected specimens of 17 colorectal cancer patients were fragmented and cultured, and the supernatant was collected. A human umbilical endothelial cell line (EA-hy 926 cells) was incubated with the supernatant. The proliferative activity was examined and the levels of basic FGF and VEGF were measured. The activities were found to be significantly related to VEGF, the depth of tumor invasion and the tumor stage.
MeSH terms
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Adult
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Aged
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Cell Division / drug effects*
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Colorectal Neoplasms / metabolism*
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Colorectal Neoplasms / pathology
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Culture Media
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Endothelial Growth Factors / biosynthesis
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Endothelial Growth Factors / pharmacology*
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Endothelium, Vascular / cytology*
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Endothelium, Vascular / drug effects
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Enzyme-Linked Immunosorbent Assay
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Female
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Fibroblast Growth Factor 2 / biosynthesis
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Fibroblast Growth Factor 2 / pharmacology
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Humans
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Lymphokines / biosynthesis
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Lymphokines / pharmacology*
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Male
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Middle Aged
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Neoplasm Staging
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Tumor Cells, Cultured
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Umbilical Veins
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Vascular Endothelial Growth Factor A
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Vascular Endothelial Growth Factors
Substances
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Culture Media
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Endothelial Growth Factors
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Lymphokines
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Vascular Endothelial Growth Factor A
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Vascular Endothelial Growth Factors
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Fibroblast Growth Factor 2