Abstract
All of the transmembrane glutamates of Tet(L) are essential for tetracycline (TET) resistance, and E397 has been shown to be essential for all catalytic modes, i.e., TET-Me(2+) and Na(+) efflux and K(+) uptake. Loop residues D74 and G70 are essential for TET flux but not for Na(+) or K(+) flux. A cysteineless Tet(L) protein exhibits all activities.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antiporters / chemistry
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Antiporters / genetics*
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Antiporters / metabolism
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Cysteine / genetics*
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Escherichia coli / metabolism
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Escherichia coli Proteins*
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Glutamates / metabolism
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Ion Transport
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Membrane Proteins / chemistry
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Membrane Proteins / genetics*
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Membrane Proteins / metabolism
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Mutagenesis, Site-Directed
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Potassium / metabolism
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Sodium / metabolism
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Tetracycline Resistance / genetics
Substances
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Antiporters
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Escherichia coli Proteins
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Glutamates
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Membrane Proteins
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Tet(L) protein, E coli
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Sodium
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Cysteine
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Potassium