Abstract
Progression through vertebrate oocyte maturation requires that pre-existing, maternally derived mRNAs be translated in a strict temporal order. The mechanism that controls the timing of oocyte mRNA translation is unknown. In this study we show that the early translational induction of the mRNA encoding the Mos proto-oncogene is mediated through a novel regulatory element within the 3' untranslated region of the Mos mRNA. This novel element is responsive to the MAP kinase signaling pathway and is distinct from the late acting, cdc2-responsive, cytoplasmic polyadenylation element. Our findings suggest that the timing of maternal mRNA translation is controlled through signal transduction pathways targeting distinct 3' UTR mRNA elements.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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3' Untranslated Regions
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Animals
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Base Sequence
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Blotting, Northern
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Blotting, Western
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CDC2 Protein Kinase / metabolism
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Cytoplasm / metabolism
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Glutathione Transferase / metabolism
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MAP Kinase Signaling System
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Models, Biological
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Molecular Sequence Data
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Mutagenesis
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Oocytes / metabolism
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Plasmids / metabolism
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Polyadenylation
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Progesterone / metabolism
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Protein Biosynthesis
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Proto-Oncogene Proteins c-mos / metabolism*
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Proto-Oncogene Proteins c-mos / physiology
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RNA / metabolism
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RNA, Messenger / metabolism
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Recombinant Fusion Proteins / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Signal Transduction
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Time Factors
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Xenopus / metabolism*
Substances
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3' Untranslated Regions
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RNA, Messenger
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Recombinant Fusion Proteins
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Progesterone
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RNA
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Glutathione Transferase
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Proto-Oncogene Proteins c-mos
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CDC2 Protein Kinase