Abstract
By homologous recombination of an internal ribosome entry site and Cre recombinase coding region into the 3'-untranslated region of the mouse Emx1 gene, we have generated a strain of mice, Emx1(IRES)cre, that expresses the Cre recombinase in a spatial and temporal pattern like that observed for Emx1. When mated to reporter strains, these mice are a sensitive means to fate-map the Emx1-expressing cells of the developing forebrain. Our results demonstrate that radial glia, Cajal-Retzius cells, glutamatergic neurons, astrocytes, and oligodendrocytes of most pallial structures originate from an Emx1-expressing lineage. On the other hand, most of the pallial GABAergic neurons arise outside the Emx1-expressing lineage. Structures that are located near the basal ganglia (e.g., the amygdala and endopiriform nuclei) are not uniformly derived from Emx1-expressing cells.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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3' Untranslated Regions / genetics
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Amygdala / cytology
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Amygdala / embryology
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Animals
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Basal Ganglia / cytology
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Basal Ganglia / embryology
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Cell Lineage
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Cerebral Cortex / cytology
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Cerebral Cortex / embryology
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Gene Targeting
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Genes, Reporter
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Homeodomain Proteins / biosynthesis*
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Homeodomain Proteins / genetics
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Integrases / biosynthesis
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Integrases / genetics
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Interneurons / cytology
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Mice
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Mice, Mutant Strains
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Models, Animal
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Neuroglia / cytology*
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Neurons / cytology*
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Neurons / metabolism
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Recombination, Genetic
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Stem Cells / cytology
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Stem Cells / metabolism
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Telencephalon / cytology*
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Telencephalon / embryology*
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Transcription Factors
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Viral Proteins / biosynthesis
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Viral Proteins / genetics
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gamma-Aminobutyric Acid / metabolism*
Substances
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3' Untranslated Regions
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Homeodomain Proteins
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Transcription Factors
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Viral Proteins
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empty spiracles homeobox proteins
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gamma-Aminobutyric Acid
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Cre recombinase
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Integrases