Gene dosage and linezolid resistance in Enterococcus faecium and Enterococcus faecalis

S H Marshall; C J Donskey; R Hutton-Thomas; R A Salata; L B Rice

doi:10.1128/AAC.46.10.3334-3336.2002

Gene dosage and linezolid resistance in Enterococcus faecium and Enterococcus faecalis

Antimicrob Agents Chemother. 2002 Oct;46(10):3334-6. doi: 10.1128/AAC.46.10.3334-3336.2002.

Authors

S H Marshall¹, C J Donskey, R Hutton-Thomas, R A Salata, L B Rice

Affiliation

¹ Research and Medical Services, Department of Veterans Affairs Medical Center and Division of Infectious Diseases, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA.

Abstract

Resistance to linezolid has been associated with a G2576U mutation in domain V of the 23S rRNA. We analyzed nine clinical isolates of linezolid-resistant enterococci and showed a clear association between the number of 23S rRNA genes containing this mutation and the level of linezolid resistance expressed.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Acetamides / pharmacology*
Drug Resistance, Bacterial / genetics*
Enterococcus faecalis / drug effects*
Enterococcus faecalis / genetics
Enterococcus faecium / drug effects*
Enterococcus faecium / genetics
Gene Dosage*
Genes, rRNA
Humans
Linezolid
Mutation
Oxazolidinones / pharmacology*
Polymerase Chain Reaction
RNA, Ribosomal, 23S / genetics

Substances

Acetamides
Oxazolidinones
RNA, Ribosomal, 23S
Linezolid