Abstract
The class II transactivator (CIITA) is the master regulator of major histocompatibility complex class II (MHCII) transcription. Its activity is regulated at the post-transcriptional level by phosphorylation and oligomerization. This aggregation mapped to and depended on the phosphorylation of residues between positions 253 and 321 in CIITA, which resulted in a dramatic accumulation of the protein and increased expression of MHCII genes in human promonocytic U937 cells, which represent immature antigen-presenting cells. Thus, the post-transcriptional modification of CIITA plays an important role in the immune response.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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COS Cells
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Cell Nucleus / drug effects
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Cell Nucleus / metabolism
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Cytoplasm / drug effects
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Cytoplasm / metabolism
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Gene Expression
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Genes, MHC Class II*
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Humans
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Nuclear Proteins*
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Phosphorylation
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Promoter Regions, Genetic
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Protein Processing, Post-Translational
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Protein Structure, Quaternary
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Protein Structure, Tertiary
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Sequence Deletion
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Temperature
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Tetradecanoylphorbol Acetate / pharmacology
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Trans-Activators / chemistry
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Trans-Activators / genetics
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Trans-Activators / metabolism*
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Transfection
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U937 Cells
Substances
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MHC class II transactivator protein
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Nuclear Proteins
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Recombinant Proteins
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Trans-Activators
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Tetradecanoylphorbol Acetate