Human rhinovirus 87 identified as human enterovirus 68 by VP4-based molecular diagnosis

Intervirology. 2002;45(3):136-41. doi: 10.1159/000065866.

Abstract

Human rhinoviruses (HRVs) are the major cause of respiratory infections. We developed a diagnostic method for HRVs based on the reverse-transcription polymerase chain reaction (RT-PCR) and VP4-based phylogenetic analysis. A set of primers used in the RT-PCR of human enteroviruses (EVs) appeared to be capable of amplifying all prototype strains of HRVs, each of which generated a 530-bp fragment. The single exception was HRV-87, which generated a 650-bp fragment, as observed in human EVs. The VP4 nucleotide sequence of HRV-87 showed more than 97% nucleotide identity with human EV-68, and formed a monophyletic cluster along with the prototype strain of EV-68 in the human EV-D cluster. HRV-87 showed the second highest homology (76.8%) with EV-70, another member of the human EV-D, in a sample of 66 human EVs and 12 HRVs. Therefore, HRV-87 should be reclassified into the cluster containing human EV-68.

MeSH terms

  • Capsid Proteins / chemistry
  • Capsid Proteins / genetics
  • Capsid Proteins / metabolism
  • Enterovirus / classification*
  • Enterovirus / genetics*
  • Enterovirus D, Human / chemistry
  • Enterovirus D, Human / genetics
  • Enterovirus Infections / diagnosis*
  • Enterovirus Infections / virology
  • Humans
  • Molecular Sequence Data
  • Phylogeny*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Rhinovirus / classification*
  • Rhinovirus / genetics*
  • Sequence Analysis, DNA

Substances

  • Capsid Proteins