Synthesis of 6'-sulfodisaccharides by beta-N-acetylhexosaminidase-catalyzed transglycosylation

Hirotaka Uzawa; Xiaoxiong Zeng; Norihiko Minoura

doi:10.1039/b209893h

Synthesis of 6'-sulfodisaccharides by beta-N-acetylhexosaminidase-catalyzed transglycosylation

Chem Commun (Camb). 2003 Jan 7:(1):100-1. doi: 10.1039/b209893h.

Authors

Hirotaka Uzawa¹, Xiaoxiong Zeng, Norihiko Minoura

Affiliation

¹ Laboratory of Advanced Bioelectronics, National Institute of Advanced Industrial Science and Technology (AIST), Central 5, 1-1-1 Higashi, Tsukuba 305-8565, Japan. h.uzawa@aist.go.jp

PMID: 12610985
DOI: 10.1039/b209893h

Abstract

Presulfated N-acetylglucosaminyl donor (pNP beta-D-6-SO3-GlcNAc) was applied for the synthesis of sulfosugars using the beta-N-acetylhexosaminidase-catalyzed transglycosylation, to afford the critically stereocontrolled sulfodisaccharides carrying the 6-sulfo GlcNAc residue at the non-reducing sides in one step.

MeSH terms

Aspergillus / enzymology
Catalysis
Chromatography, High Pressure Liquid
Disaccharides / chemical synthesis*
Disaccharides / chemistry
Glycosylation
Magnetic Resonance Spectroscopy
Molecular Structure
Sulfuric Acid Esters / chemistry
beta-N-Acetylhexosaminidases / metabolism*

Substances

Disaccharides
Sulfuric Acid Esters
beta-N-Acetylhexosaminidases