Abstract
Paenibacillus polymyxa ATCC 12321 produced more acetic acid and less butanediol from xylose than from glucose. The product yields from xylose were ethanol (0.72 mol/mol sugar), (R,R)-2,3-butanediol (0.31 mol/mol sugar), and acetate (0.38 mol/mol sugar) while those from glucose were ethanol (0.74 mol/mol sugar), (R,R)-2,3-butanediol (0.46 mol/mol sugar), and acetate (0.05 mol/mol sugar). Higher acetate kinase activity and lower acetate uptake ability were found in xylose-grown cells than in glucose-grown cells. Furthermore, phosphoketolase activity was higher in xylose-grown cells than in glucose-grown cells. In fed-batch culture on xylose, glucose feeding raised the butanediol yield to 0.56 mol/mol sugar and reduced acetate accumulation to 0.04 mol/mol sugar.
MeSH terms
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Acetate Kinase / metabolism*
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Acetates / analysis*
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Acetates / metabolism
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Acetoin / analysis
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Aldehyde-Lyases / metabolism*
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Bacillus / metabolism
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Biomass
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Butylene Glycols / analysis
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Butylene Glycols / metabolism
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Carbon Dioxide / analysis
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Culture Media / chemistry
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Ethanol / analysis
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Ethanol / metabolism
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Fermentation
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Formates / analysis
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Glucose / metabolism
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Glycolysis
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Gram-Positive Endospore-Forming Bacteria / metabolism*
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Hydrogen / analysis
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Hydrogen-Ion Concentration
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Kinetics
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Lactic Acid / analysis
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Pentose Phosphate Pathway
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Succinic Acid / analysis
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Xylose / metabolism*
Substances
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Acetates
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Butylene Glycols
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Culture Media
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Formates
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formic acid
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Carbon Dioxide
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Lactic Acid
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Ethanol
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Hydrogen
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Xylose
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Succinic Acid
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Acetoin
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Acetate Kinase
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Aldehyde-Lyases
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phosphoketolase
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Glucose