Linear phase imaging using differential interference contrast microscopy

J Microsc. 2004 Apr;214(Pt 1):7-12. doi: 10.1111/j.0022-2720.2004.01293.x.

Abstract

We propose an extension to Nomarski differential interference contrast microscopy that enables isotropic linear phase imaging. The method combines phase shifting, two directions of shear and Fourier-space integration using a modified spiral phase transform. We simulated the method using a phantom object with spatially varying amplitude and phase. Simulated results show good agreement between the final phase image and the object phase, and demonstrate resistance to imaging noise.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fourier Analysis
  • Image Processing, Computer-Assisted
  • Microscopy, Phase-Contrast / methods*
  • Models, Theoretical