Abstract
In this paper we report the isolation and structural elucidation of a new flavonoid (1) and three known compounds, 6,8-di-C-methylkaempferol 3-methyl ether (2), oleanolic acid, and 2 alpha,3 beta-dihydroxyurs-12-en-28-oic acid, from aerial parts of Eucalyptus occidentalis collected in Algeria. Flavonoids 1 and 2 were used to study their biological activities on the human promyelocytic leukemia cell line, HL-60. Our data show that these compounds induce morphological changes and internucleosomal DNA fragmentation characteristic of apoptotic cell death, which is mediated by caspase-8/caspase-3 activation and cytochrome c release.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Algeria
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Antineoplastic Agents / chemistry
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Antineoplastic Agents / isolation & purification*
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Antineoplastic Agents / pharmacology
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Apoptosis / drug effects*
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Caspase 3
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Caspase 8
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Caspases / metabolism
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Cytochrome c Group / metabolism
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DNA Fragmentation
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Enzyme Activation
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Eucalyptus
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Flavonoids / chemistry
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Flavonoids / isolation & purification*
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Flavonoids / pharmacology
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HL-60 Cells / chemistry*
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Humans
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Kaempferols / chemistry
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Kaempferols / isolation & purification*
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Kaempferols / pharmacology*
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Leukemia, Myeloid
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Molecular Structure
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Oleanolic Acid / pharmacology
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Plant Leaves / chemistry
Substances
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6,8-di-C-methylkaempferol 3-methyl ether
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Antineoplastic Agents
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Cytochrome c Group
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Flavonoids
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Kaempferols
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cytochrome c''
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Oleanolic Acid
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kaempferol
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CASP3 protein, human
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CASP8 protein, human
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Caspase 3
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Caspase 8
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Caspases