Abstract
An unknown yeast species was isolated from maize silage and was determined to be novel on the basis of morphological and physiological characteristics, nucleotide sequence of domain D1/D2 of LSU rDNA and from its electrophoretic karyotype. The name for the proposed new species is Saccharomyces bulderi Middelhoven, Kurtzman et Vaughan-Martini (type strain CBS 8638, NRRL Y-27203, DBVPG 7127). S. bulderi is closely related to S. barnettii and S. exiguus from which it can be distinguished by having a double vitamin requirement of biotin and thiamine and by no or slow aerobic growth on raffinose, a sugar that on the contrary is fermented rapidly. Gluconolactone is rapidly fermented with ethanol, glycerol and carbon dioxide being the main products.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aerobiosis
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Biotin / metabolism
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Carbon Dioxide / metabolism
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DNA, Fungal / analysis
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DNA, Fungal / chemistry
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DNA, Fungal / isolation & purification
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DNA, Ribosomal / chemistry
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DNA, Ribosomal / isolation & purification
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Fermentation
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Genes, rRNA
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Gluconates / metabolism*
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Glycerol / metabolism
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Karyotyping
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Lactones
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Methanol / metabolism
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Molecular Sequence Data
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Mycological Typing Techniques
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RNA, Ribosomal / genetics
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Raffinose / metabolism
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Saccharomyces / classification*
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Saccharomyces / cytology
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Saccharomyces / isolation & purification*
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Saccharomyces / metabolism
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Sequence Analysis, DNA
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Thiamine / metabolism
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Zea mays / microbiology
Substances
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DNA, Fungal
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DNA, Ribosomal
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Gluconates
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Lactones
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RNA, Ribosomal
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RNA, ribosomal, 26S
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Carbon Dioxide
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Biotin
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Raffinose
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Glycerol
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beta-glucono-1,5-lactone
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Thiamine
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Methanol