Quantification of Fusarium graminearum in infected wheat by species specific real-time PCR applying a TaqMan Probe

G H Reischer; M Lemmens; A Farnleitner; A Adler; R L Mach

doi:10.1016/j.mimet.2004.06.003

Quantification of Fusarium graminearum in infected wheat by species specific real-time PCR applying a TaqMan Probe

J Microbiol Methods. 2004 Oct;59(1):141-6. doi: 10.1016/j.mimet.2004.06.003.

Authors

G H Reischer¹, M Lemmens, A Farnleitner, A Adler, R L Mach

Affiliation

¹ Institute for Chemical Engineering, Gene Technology Group, Vienna University of Technology, Getreidemarkt 9/166, A-1060 Vienna, Austria.

PMID: 15325762
DOI: 10.1016/j.mimet.2004.06.003

Abstract

A new real-time PCR based method was developed for the species-specific detection, identification and quantification of Fusarium graminearum in planta. It utilizes a TaqMan hybridisation probe targeting the beta-tubulin gene and a plasmid standard. The assay is highly specific giving no product with DNA of closely related species. It is very sensitive, detecting down to five gene copies per reaction, and is able to produce reliable quantitative data over a range of six orders of magnitude.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA, Fungal / chemistry
DNA, Fungal / genetics
Fusarium / genetics*
Plant Diseases / microbiology*
Polymerase Chain Reaction / methods*
Taq Polymerase / metabolism
Triticum*
Tubulin / chemistry
Tubulin / genetics

Substances

DNA, Fungal
Tubulin
Taq Polymerase