Development of immunocapture reverse transcription loop-mediated isothermal amplification for the detection of tomato spotted wilt virus from chrysanthemum

J Virol Methods. 2004 Oct;121(1):49-55. doi: 10.1016/j.jviromet.2004.05.016.

Abstract

An immunocapture reverse transcription loop-mediated isothermal amplification (IC/RT-LAMP) was developed for the detection of tomato spotted wilt virus (TSWV) from chrysanthemum. This method enabled sensitive, reproducible and specific detection of TSWV from chrysanthemum plants. In the RT-LAMP method, TSWV genomic RNA could be amplified under isothermal (65 degrees C) conditions within 1 h. The resulting amplicons were detected by the measurement or observation of the turbidity of the reaction mixture without gel electrophoresis. IC/RT-LAMP was 100 times more sensitive than IC/RT-PCR.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Base Sequence
  • Chrysanthemum / virology*
  • Molecular Sequence Data
  • Nephelometry and Turbidimetry
  • Nucleic Acid Amplification Techniques / methods*
  • Plant Diseases / virology*
  • RNA, Viral / analysis
  • RNA, Viral / isolation & purification
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Tospovirus / genetics
  • Tospovirus / isolation & purification*

Substances

  • RNA, Viral

Associated data

  • GENBANK/AB010996