Binding of pro-matrix metalloproteinase 9 by Fusobacterium nucleatum subsp. nucleatum as a mechanism to promote the invasion of a reconstituted basement membrane

Infect Immun. 2004 Oct;72(10):6160-3. doi: 10.1128/IAI.72.10.6160-6163.2004.

Abstract

In this study, we investigated the ability of Fusobacterium nucleatum subsp. nucleatum to increase its tissue-invasive potential by acquiring cell-associated human matrix metalloproteinase 9 (MMP-9) activity. Binding of pro-MMP-9 to fusobacteria was demonstrated by enzyme-linked immunosorbent assay. Zymography and a colorimetric assay showed that bound pro-MMP-9 can be converted into a proteolytically active form. The potential contribution of this acquired host activity in tissue invasion was demonstrated using a reconstituted basement membrane (Matrigel).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Basement Membrane / metabolism*
  • Basement Membrane / microbiology*
  • Collagen / metabolism*
  • Collagenases / metabolism*
  • Colorimetry
  • Drug Combinations
  • Enzyme Activation
  • Enzyme Precursors / metabolism*
  • Enzyme-Linked Immunosorbent Assay
  • Fusobacterium nucleatum / metabolism*
  • Humans
  • Laminin / metabolism*
  • Matrix Metalloproteinase 9 / metabolism*
  • Protein Binding
  • Proteoglycans / metabolism*

Substances

  • Drug Combinations
  • Enzyme Precursors
  • Laminin
  • Proteoglycans
  • matrigel
  • Collagen
  • Collagenases
  • pro-matrix metalloproteinase 9
  • Matrix Metalloproteinase 9