Abstract
In this study, we examined the effect of hydrogen peroxide on the accumulation of various mRNAs encoding heat shock proteins (hsps) and proto-oncogenes in Xenopus A6 kidney epithelial cells. Hydrogen peroxide treatment enhanced the accumulation of hsp90, hsp70, hsp30, c-jun, c-fos, and actin mRNAs with distinct temporal patterns. Although hsp70, c-fos, and c-jun mRNA levels peaked at 1-2 h before declining, hsp30 and hsp90 mRNA levels were maximal at 4-6 h. Other mRNAs, including heat shock cognate hsc70, immunoglobulin binding protein, and ribosomal L8, were unaffected. Treatment of kidney cells with a combination of mild heat shock plus hydrogen peroxide resulted in a synergistic increase in the relative levels of both hsp70 and hsp30 mRNA, but not hsp90, c-fos, c-jun, or actin. This study suggests that analysis of hsp and proto-oncogene mRNA levels may be of value as molecular biomarkers of oxidative stress associated with various disease states and nephrotoxicity in kidney.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Actins / biosynthesis
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Actins / genetics
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Animals
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Biomarkers
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Cells, Cultured
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Epithelial Cells / drug effects
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Epithelial Cells / metabolism
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HSP30 Heat-Shock Proteins
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HSP70 Heat-Shock Proteins / biosynthesis
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HSP70 Heat-Shock Proteins / genetics
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HSP90 Heat-Shock Proteins / biosynthesis
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HSP90 Heat-Shock Proteins / genetics
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Heat-Shock Proteins / biosynthesis*
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Heat-Shock Proteins / genetics
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Hydrogen Peroxide / pharmacology*
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Kidney / cytology
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Kidney / drug effects
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Kidney / metabolism*
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Membrane Proteins / biosynthesis
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Membrane Proteins / genetics
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Oxidants / pharmacology*
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Oxidative Stress
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Proto-Oncogene Proteins c-fos / biosynthesis*
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Proto-Oncogene Proteins c-fos / genetics
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Proto-Oncogene Proteins c-jun / biosynthesis*
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Proto-Oncogene Proteins c-jun / genetics
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RNA, Messenger / biosynthesis*
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Xenopus Proteins
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Xenopus laevis
Substances
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Actins
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Biomarkers
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HSP30 Heat-Shock Proteins
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HSP70 Heat-Shock Proteins
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HSP90 Heat-Shock Proteins
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Heat-Shock Proteins
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Membrane Proteins
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Oxidants
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Proto-Oncogene Proteins c-fos
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Proto-Oncogene Proteins c-jun
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RNA, Messenger
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Xenopus Proteins
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hsp30 protein, Xenopus
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Hydrogen Peroxide