Probing proton-proton proximities in the solid state: high-resolution two-dimensional 1H-1H double-quantum CRAMPS NMR spectroscopy

Steven P Brown; Anne Lesage; Bénédicte Elena; Lyndon Emsley

doi:10.1021/ja045461p

Probing proton-proton proximities in the solid state: high-resolution two-dimensional 1H-1H double-quantum CRAMPS NMR spectroscopy

J Am Chem Soc. 2004 Oct 20;126(41):13230-1. doi: 10.1021/ja045461p.

Authors

Steven P Brown¹, Anne Lesage, Bénédicte Elena, Lyndon Emsley

Affiliation

¹ Department of Physics, University of Warwick, Coventry CV4 7AL, United Kingdom. s.p.brown@warwick.ac.uk

PMID: 15479070
DOI: 10.1021/ja045461p

Abstract

A new 1H DQ (double-quantum) CRAMPS (combined rotation and multiple-pulse sequence) solid-state nuclear magnetic resonance experiment incorporating DUMBO homonuclear 1H dipolar decoupling is presented. The major resolution enhancement enables DQ peaks corresponding to all 22 close (<3.5 A) proton-proton proximities in the dipeptide beta-AspAla to be observed. In particular, the DQ CRAMPS spectrum provides access to the alkyl region of the spectrum and yields a clear assignment of the two CH and two diastereotopic CH2 proton resonances.