Abstract
The forkhead transcription factor Fkh2p acts in a DNA-bound complex with Mcm1p and the coactivator Ndd1p to regulate cell cycle-dependent expression of the CLB2 gene cluster in Saccharomyces cerevisiae. Here, we demonstrate that Fkh2p is a target of cyclin-dependent protein kinases and that phosphorylation of Fkh2p promotes interactions between Fkh2p and the coactivator Ndd1p. These phosphorylation-dependent changes in the Fkh2p-Ndd1p complex play an important role in the cell cycle-regulated expression of the CLB2 cluster. Our data therefore identify an important regulatory target for cyclin-dependent kinases in the cell cycle and further our molecular understanding of the key cell cycle regulatory transcription factor Fkh2p.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Binding Sites
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CDC28 Protein Kinase, S cerevisiae / genetics
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CDC28 Protein Kinase, S cerevisiae / metabolism
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Cell Cycle
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Cell Cycle Proteins / genetics
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Cell Cycle Proteins / metabolism*
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Cyclin B / genetics
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Cyclin B / metabolism
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Cyclin-Dependent Kinases / genetics
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Cyclin-Dependent Kinases / metabolism*
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Forkhead Transcription Factors
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Gene Expression Regulation, Fungal
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Genes, Fungal
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Macromolecular Substances
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Models, Biological
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Multigene Family
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Mutagenesis
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Phosphorylation
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Saccharomyces cerevisiae / cytology
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism*
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Transcription Factors / genetics
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Transcription Factors / metabolism*
Substances
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CLB2 protein, S cerevisiae
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Cell Cycle Proteins
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Cyclin B
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Fkh2 protein, S cerevisiae
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Forkhead Transcription Factors
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Macromolecular Substances
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NDD1 protein, S cerevisiae
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Recombinant Fusion Proteins
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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CDC28 Protein Kinase, S cerevisiae
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Cyclin-Dependent Kinases