Selective preparation of N-acetyl-D-glucosamine and N,N'-diacetylchitobiose from chitin using a crude enzyme preparation from Aeromonas sp

Ju Hee Kuk; Woo Jin Jung; Gyung Hyun Jo; Joon Seob Ahn; Kil Yong Kim; Ro Dong Park

doi:10.1007/s10529-004-6300-3

Selective preparation of N-acetyl-D-glucosamine and N,N'-diacetylchitobiose from chitin using a crude enzyme preparation from Aeromonas sp

Biotechnol Lett. 2005 Jan;27(1):7-11. doi: 10.1007/s10529-004-6300-3.

Authors

Ju Hee Kuk¹, Woo Jin Jung, Gyung Hyun Jo, Joon Seob Ahn, Kil Yong Kim, Ro Dong Park

Affiliation

¹ Glucosamine Saccharide Materials-National Research Laboratory (GSM-NRL), Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Chonnam National University, 500-757, Gwangju, Korea.

PMID: 15685412
DOI: 10.1007/s10529-004-6300-3

Abstract

A bacterium, Aeromonas sp. GJ-18, having strong chitinolytic activity was isolated from coastal soil and used for crude enzyme preparations. This enzyme preparation contained N-acetyl-D-glucosaminidase and N,N'-diacetylchitobiohydrolase. N-Acetyl-D-glucosaminidase was inactive above 50 degrees C, but N,N'-diacetylchitobiohydrolase was stable at this temperature. Utilizing the temperature sensitivities of the chitin degradation enzymes in crude enzyme preparation, N-acetyl-D-glucosamine (GlcNAc) and N,N'-diacetylchitobiose [(GlcNAc)(2)] were selectively produced from chitin. At 45 degrees C, GlcNAc was produced as a major hydrolytic product (94% composition) with a yield of 74% in 5 d, meanwhile at 55 degrees C (GlcNAc)(2) was the major product (86%) with a yield of 35% within 5 d.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetylglucosamine / biosynthesis*
Aeromonas / enzymology*
Chitin / metabolism*
Disaccharides / biosynthesis*
Glucosidases / metabolism*
Hydrolysis

Substances

Disaccharides
Chitin
N,N-diacetylchitobiose
Glucosidases
N,N'-diacetylchitobiohydrolase, Aeromonas
Acetylglucosamine