A sensitive method for the detection of proteins by high-efficiency fluorescence quenching

Analyst. 2005 Mar;130(3):283-5. doi: 10.1039/b414239j. Epub 2005 Jan 19.

Abstract

A successful method for the detection of electron transfer proteins such as cytochrome c, hemoglobin and myoglobin has been developed based on the fluorescence quenching of semiconductor nanocrystals. High sensitivity and a good linear relationship are obtained.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cadmium Compounds
  • Electron Transport
  • Fluorescence Resonance Energy Transfer / methods
  • Hemeproteins / analysis*
  • Nanostructures
  • Selenium Compounds

Substances

  • Cadmium Compounds
  • Hemeproteins
  • Selenium Compounds
  • cadmium selenide