Abstract
A new cyclic nitropeptide, psychrophilin D (1), together with two known cyclic peptides, cycloaspeptide A (2) and cycloaspeptide D (3), were isolated from the psychrotolerant fungus Penicillium algidum using C18 flash chromatography, LH-20 Sephadex and preparative HPLC. The structure of psychrophilin D (1) was derived from mass spectrometric information, 1D and 2D NMR spectra and Marfey's method. The compounds were tested in antimicrobial, antiviral, anticancer and antiplasmodial assays. Psychrophilin D (1) exhibited a moderate activity (ID50 10.1 microg/ml) in the P388 murine leukaemia cell assay. Cycloaspeptide A (2) and D (3) exhibited moderate activity (IC50 3.5 and 4.7 microg/ml, respectively) against Plasmodium falciparum.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Bacterial Agents / isolation & purification
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Anti-Bacterial Agents / pharmacology*
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Antibiotics, Antineoplastic / pharmacology
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Antimalarials / pharmacology
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Antiviral Agents / pharmacology
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Bacteria / drug effects
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Cell Line, Tumor
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Chromatography, High Pressure Liquid
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Circular Dichroism
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Drug Screening Assays, Antitumor
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Humans
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Leukemia P388 / drug therapy
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Magnetic Resonance Spectroscopy
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Mass Spectrometry
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Mice
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Microbial Sensitivity Tests
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Penicillium / chemistry*
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Peptides, Cyclic / isolation & purification
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Peptides, Cyclic / pharmacology*
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Plasmodium falciparum / drug effects
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Viruses / drug effects
Substances
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Anti-Bacterial Agents
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Antibiotics, Antineoplastic
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Antimalarials
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Antiviral Agents
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Peptides, Cyclic
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cycloaspeptide A
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psychrophilin D