Phaseococcin, an antifungal protein with antiproliferative and anti-HIV-1 reverse transcriptase activities from small scarlet runner beans

Biochem Cell Biol. 2005 Apr;83(2):212-20. doi: 10.1139/o05-037.

Abstract

From the seeds of small scarlet runner beans (Phaseolus coccineus 'Minor'), an antifungal protein with an N-terminal sequence homologous to those of defensins was isolated. The antifungal protein bound to Affi-gel blue gel and Mono S but it did not bind to DEAE-cellulose. It was further purified by gel filtration on a Superdex peptide column. It exhibited a molecular mass of 5422 Da as determined by mass spectrometry. The protein, designated as phaseococcin, suppressed mycelial growth in a number of fungi including Botrytis cinerea, Coprinus comatus, Fusarium oxysporum, Mycosphaerella arachidicola, Physalospora piricola, and Rhizoctonia solani. It also inhibited proliferation in several Bacillus species and the leukemia cell lines HL60 and L1210 and curtailed the activity of HIV-1 reverse transcriptase. It did not affect proliferation of mouse splenocytes and neither did it inhibit protein synthesis in a cell-free rabbit reticulocyte lysate system.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Anti-Bacterial Agents / isolation & purification
  • Anti-Bacterial Agents / pharmacology
  • Antifungal Agents / isolation & purification*
  • Antifungal Agents / pharmacology
  • Antineoplastic Agents / isolation & purification
  • Antineoplastic Agents / pharmacology*
  • Bacteria / drug effects
  • Cell Proliferation / drug effects
  • Cell-Free System
  • Chromatography, Affinity
  • Chromatography, Gel
  • Chromatography, Ion Exchange
  • Defensins / isolation & purification
  • Defensins / pharmacology*
  • Fabaceae / chemistry*
  • Fungi / drug effects
  • HIV Reverse Transcriptase / antagonists & inhibitors*
  • HIV Reverse Transcriptase / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Peptide Fragments / isolation & purification
  • Peptide Fragments / pharmacology*
  • Phaseolus / chemistry
  • Proteins / metabolism
  • Proteins / pharmacology
  • Rabbits
  • Reverse Transcriptase Inhibitors / isolation & purification*
  • Reverse Transcriptase Inhibitors / pharmacology
  • Seeds / chemistry
  • Sequence Homology, Amino Acid
  • Spleen / cytology
  • Spleen / drug effects
  • Tumor Cells, Cultured

Substances

  • Anti-Bacterial Agents
  • Antifungal Agents
  • Antineoplastic Agents
  • Defensins
  • Peptide Fragments
  • Proteins
  • Reverse Transcriptase Inhibitors
  • phaseococcin protein, Phaseolus coccineus
  • HIV Reverse Transcriptase