Abstract
Target specificity for bacteriophage Mu was studied using a new phage derivative that enabled cloning of Mu-host junctions from phage DNA. Insertions distributed throughout the chromosome showed no orientation bias with respect to transcription or replication polarity. Genes with a high frequency of the triplet CGG were preferred targets.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Bacteriophage mu / genetics*
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Chromosomes, Bacterial
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Consensus Sequence
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DNA, Viral / genetics
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Escherichia coli / genetics*
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Escherichia coli / virology*
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Molecular Sequence Data
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Replication Origin / genetics
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Trinucleotide Repeats*