An aborted full-term bovine foetus bad multisystemic anomalies that included a heart defect, cleft palate, pulmonary hypoplasia and kyphoscoliosis. Karyotyping using pericardial sac fibroblast cells indicated that the foetal cells were trisomic. Trypsin-banding procedures and Giemsa staining revealed that the extra chromosome was presumptively Chromosome 27. This is the first report of chromosomal trisomy 27 in an abnormal bovine foetus, and highlights the significance of chromosomal aberrations as a cause of multi-systemic foetal anomalies.