Fluorescence resonance energy transfer between a quantum dot donor and a dye acceptor attached to DNA

Chem Commun (Camb). 2005 Oct 14:(38):4807-9. doi: 10.1039/b508911e. Epub 2005 Sep 6.

Abstract

We show that direct coupling of a dye-labelled DNA (acceptor) to a quantum dot (QD) donor significantly reduces the donor-acceptor distance and improves the FRET efficiency: a highly efficient FRET (approximately 88%) at a low acceptor-to-donor ratio of 2 has been achieved at the single-molecule level.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cadmium Compounds / chemistry
  • Coloring Agents / chemistry*
  • DNA / chemistry*
  • Fluorescence Resonance Energy Transfer / methods*
  • Quantum Dots*
  • Selenium Compounds / chemistry
  • Sensitivity and Specificity
  • Structure-Activity Relationship
  • Sulfhydryl Compounds / chemistry
  • Sulfides / chemistry
  • Time Factors
  • Zinc Compounds / chemistry

Substances

  • Cadmium Compounds
  • Coloring Agents
  • Selenium Compounds
  • Sulfhydryl Compounds
  • Sulfides
  • Zinc Compounds
  • DNA
  • cadmium selenide
  • zinc sulfide