Abstract
Mono Lake is an alkaline hypersaline lake that supports high methane oxidation rates. Retrieved pmoA sequences showed a broad diversity of aerobic methane oxidizers including the type I methanotrophs Methylobacter (the dominant genus), Methylomicrobium, and Methylothermus, and the type II methanotroph Methylocystis. Stratification of Mono Lake resulted in variation of aerobic methane oxidation rates with depth. Methanotroph diversity as determined by analysis of pmoA using new denaturing gradient gel electrophoresis primers suggested that variations in methane oxidation activity may correlate with changes in methanotroph community composition.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aerobiosis
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Ecosystem*
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Electrophoresis / methods
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Fresh Water / chemistry
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Fresh Water / microbiology*
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Hydrogen-Ion Concentration
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Methane / metabolism*
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Methylococcaceae / classification*
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Methylococcaceae / enzymology
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Methylococcaceae / genetics
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Methylocystaceae / classification*
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Methylocystaceae / enzymology
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Methylocystaceae / genetics
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Molecular Sequence Data
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Oxidation-Reduction
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Oxygenases / genetics*
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Oxygenases / metabolism
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Sequence Analysis, DNA
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Sodium Chloride
Substances
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Sodium Chloride
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Oxygenases
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methane monooxygenase
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Methane
Associated data
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GENBANK/AY571985
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GENBANK/AY571986
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GENBANK/AY571987
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GENBANK/AY571988
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GENBANK/AY571989
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GENBANK/AY571990
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GENBANK/AY571991
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GENBANK/AY571992
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GENBANK/AY571993
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GENBANK/AY571994
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GENBANK/AY571995
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GENBANK/AY571996
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GENBANK/AY571997
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GENBANK/AY571998
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GENBANK/AY571999
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GENBANK/AY572000
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GENBANK/AY572001
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GENBANK/AY572002
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GENBANK/AY572003
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GENBANK/AY572004
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GENBANK/DQ059819
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GENBANK/DQ059820
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GENBANK/DQ059821
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GENBANK/DQ059822
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GENBANK/DQ059823