Abstract
The expression of dopamine receptors was examined in purified human neutrophils, monocytes, B cells, natural killer cells and CD4+ - and CD8+ -T lymphocytes by RT-PCR. In healthy subjects, D1 and D2 receptors were not expressed in leukocytes. Real Time PCR for dopamine receptors D3 and D4 disclosed that D3 receptors are expressed in T cells and natural killer cells and D4 receptors in CD4+ -T cells. The comparison of schizophrenic patients with sex- and age-matched controls revealed a significantly higher expression of D3 receptor mRNA in T cells of schizophrenic patients, whereas D4 receptor mRNA in CD4+ -T cells was downregulated.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adult
-
B-Lymphocytes / immunology
-
B-Lymphocytes / metabolism
-
CD4-Positive T-Lymphocytes / immunology
-
CD4-Positive T-Lymphocytes / metabolism*
-
CD8-Positive T-Lymphocytes / immunology
-
CD8-Positive T-Lymphocytes / metabolism
-
Female
-
Humans
-
Killer Cells, Natural / immunology
-
Killer Cells, Natural / metabolism
-
Male
-
Monocytes / immunology
-
Monocytes / metabolism
-
Neutrophils / immunology
-
Neutrophils / metabolism
-
RNA, Messenger / analysis
-
Receptors, Dopamine D3 / immunology
-
Receptors, Dopamine D3 / metabolism*
-
Receptors, Dopamine D4 / immunology
-
Receptors, Dopamine D4 / metabolism*
-
Reverse Transcriptase Polymerase Chain Reaction
-
Schizophrenia / immunology*
-
Schizophrenia / metabolism
Substances
-
RNA, Messenger
-
Receptors, Dopamine D3
-
Receptors, Dopamine D4