We investigate the spectral properties of chaotic quantum graphs. We demonstrate that the energy-average over the spectrum of individual graphs can be traded for the functional average over a supersymmetric nonlinear -model action. This proves that spectral correlations of individual quantum graphs behave according to the predictions of Wigner-Dyson random matrix theory. We explore the stability of the universal random matrix behavior with regard to perturbations, and discuss the crossover between different types of symmetries.