Abstract
Studies on the nature and location of the methylated nucleotides in mRNA synthesized in vitro by vaccinia virus particles revealed an unusual 5'-terminal structure. Evidence that the pyrophosphate group is blocked by 7-methylguanosine and that both 2'-O-methyl-adenosine and 2'-O-methylguanosine occupy penultimate positions was presented. According to this model, the 5'-termini of vaccinia virus mRNAs are: 7MeG-5'ppp-5'GMepNp and 7MeG-5'AMepNp.
MeSH terms
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Adenosine / analogs & derivatives
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Adenosine / metabolism*
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Alkaline Phosphatase / metabolism
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Base Sequence
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Chromatography, DEAE-Cellulose
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Chromatography, Thin Layer
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Diphosphates / metabolism
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Electrophoresis
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Guanosine / analogs & derivatives
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Guanosine / metabolism*
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Methylation
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Phosphorus Radioisotopes
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RNA, Messenger / biosynthesis*
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RNA, Viral / biosynthesis*
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S-Adenosylmethionine / metabolism
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Tritium
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Vaccinia virus / metabolism*
Substances
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Diphosphates
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Phosphorus Radioisotopes
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RNA, Messenger
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RNA, Viral
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Tritium
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Guanosine
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S-Adenosylmethionine
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Alkaline Phosphatase
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Adenosine