The inhibitory effect and mechanism of luteolin 7-glucoside on rat aortic vascular smooth muscle cell proliferation

Tack-Joong Kim; Jin-Ho Kim; Yong-Ri Jin; Yeo-Pyo Yun

doi:10.1007/BF02977471

The inhibitory effect and mechanism of luteolin 7-glucoside on rat aortic vascular smooth muscle cell proliferation

Arch Pharm Res. 2006 Jan;29(1):67-72. doi: 10.1007/BF02977471.

Authors

Tack-Joong Kim¹, Jin-Ho Kim, Yong-Ri Jin, Yeo-Pyo Yun

Affiliation

¹ Department of Biomembrane and Biofunctional Chemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan.

PMID: 16491846
DOI: 10.1007/BF02977471

Abstract

The abnormal proliferation of aortic vascular smooth muscle cells (VSMCs) plays a central role in the pathogenesis of atherosclerosis and restenosis after angioplasty and possibly also in the development of hypertension. The present study was designed to examine the inhibitory effects and the mechanism of luteolin 7-glucoside (L7G) on the platelet-derived growth factor (PDGF)-BB-induced proliferation of VSMCs. L7G significantly inhibited the PDGF-BB-induced proliferation and the DNA synthesis of the VSMCs in a concentration-dependent manner. Pre-incubation of the VSMCs with L7G significantly inhibited the PDGF-BB-induced extracellular signal-regulated kinase 1/2 (ERK1/2), Akt and the phospholipase C (PLC)-gamma1 activation. However, L7G had almost no affect on the phosphorylation of PDGF-beta receptor tyrosine kinase, which was induced by PDGF-BB. These results suggest that L7G inhibits the PDGF-BB-induced proliferation of VSMCs via the blocking of PLC-gamma1, Akt, and ERK1/2 phosphorylation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Actins / metabolism
Animals
Aorta, Thoracic / cytology
Aorta, Thoracic / drug effects
Blotting, Western
Cell Proliferation / drug effects
Cells, Cultured
Glucosides / pharmacology*
Luteolin / pharmacology*
Mitogen-Activated Protein Kinases / metabolism
Models, Molecular
Muscle, Smooth, Vascular / cytology*
Muscle, Smooth, Vascular / drug effects
Phospholipase C gamma / antagonists & inhibitors
Phospholipase C gamma / metabolism
Phosphorylation
Platelet-Derived Growth Factor / pharmacology
Protein-Tyrosine Kinases / metabolism
Proto-Oncogene Proteins c-akt / metabolism
Rats
Receptors, Platelet-Derived Growth Factor / metabolism
Signal Transduction / drug effects
Thymidine / metabolism

Substances

Actins
Glucosides
Platelet-Derived Growth Factor
luteolin-7-glucoside
Protein-Tyrosine Kinases
Receptors, Platelet-Derived Growth Factor
Proto-Oncogene Proteins c-akt
Mitogen-Activated Protein Kinases
Phospholipase C gamma
Luteolin
Thymidine