Abstract
This report describes an automated method for the measurements of cellular retention and uptake of radiolabeled proteins interacting with cell-surface receptors on intact cancer cells. A complete uptake and retention measurement was performed in one cell dish using a rotating radioimmunoassay (RIA) principle. Compared to common manual measurements, rotating RIA saved both labor time and reagents and provided real-time binding traces with superior time-resolution. The rotating RIA retention profiles for different interactions agreed with retention times reported in the literature.
MeSH terms
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Animals
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Antibodies / immunology
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Antibodies / metabolism*
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Antigens, Surface / immunology
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Antigens, Surface / metabolism*
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Carcinoma / diagnostic imaging
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Carcinoma / metabolism*
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Cell Culture Techniques / instrumentation*
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Cell Culture Techniques / methods
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Cell Line, Tumor
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Equipment Design
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Equipment Failure Analysis
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Humans
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Metabolic Clearance Rate
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Radioimmunoassay / instrumentation*
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Radioimmunoassay / methods
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Radioisotope Dilution Technique*
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Radionuclide Imaging
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Reproducibility of Results
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Robotics / instrumentation*
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Robotics / methods
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Sensitivity and Specificity
Substances
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Antibodies
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Antigens, Surface