A selective activity-based probe for the papain family cysteine protease dipeptidyl peptidase I/cathepsin C

Fang Yuan; Steven H L Verhelst; Galia Blum; Lisa M Coussens; Matthew Bogyo

doi:10.1021/ja060835v

A selective activity-based probe for the papain family cysteine protease dipeptidyl peptidase I/cathepsin C

J Am Chem Soc. 2006 May 3;128(17):5616-7. doi: 10.1021/ja060835v.

Authors

Fang Yuan¹, Steven H L Verhelst, Galia Blum, Lisa M Coussens, Matthew Bogyo

Affiliation

¹ Department of Pathology, and Microbiology & Immunology, Stanford School of Medicine, 300 Pasteur Drive, Stanford, California 94305, USA.

PMID: 16637611
DOI: 10.1021/ja060835v

Abstract

Dipeptidyl peptidase I is involved in the activation of a number of disease-related proteases by removal of N-terminal prodipeptides. We here report a selective activity-based probe for monitoring dipeptidyl peptidase I activity in whole proteomes as well as in intact cells, without labeling of closely related enzyme family members.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Cathepsin C / chemistry
Cathepsin C / metabolism*
Cell Line, Tumor
Chromatography, High Pressure Liquid
Mice
Molecular Probes*

Substances

Molecular Probes
Cathepsin C

Abstract

Publication types

MeSH terms

Substances

Grants and funding