Abstract
Arabidopsis thaliana CEL1 protein was detected in young expanding tissues. Immunostaining revealed that CEL1 accumulated mostly in xylem cells. The primary, as well as the secondary xylem showed considerable CEL1 staining. CEL1 was also observed in young epidermal cells, in which the thicker lateral and tangential walls stained more intensely than the inner walls. In newly formed cell walls, the lateral tangential walls were labeled more intensively than the inner walls. Cellulase activity was found to be significantly higher in growing tissue compared to mature parts of the plant. Cel1 expression concurrently with cellulase activity could be restored in detached matured leaves by sucrose treatment after 48 h in the culture medium.
MeSH terms
-
Antibodies / immunology
-
Arabidopsis / anatomy & histology*
-
Arabidopsis / enzymology*
-
Arabidopsis / genetics
-
Arabidopsis / growth & development
-
Arabidopsis Proteins / metabolism*
-
Blotting, Western
-
Cell Wall / metabolism*
-
Cellulase / metabolism*
-
Cellulose 1,4-beta-Cellobiosidase / metabolism*
-
Glucuronidase / metabolism
-
Plant Leaves / drug effects
-
Plant Leaves / enzymology
-
Plant Roots / cytology
-
Plant Roots / drug effects
-
Plant Roots / enzymology
-
Plant Stems / cytology
-
Plant Stems / drug effects
-
Plant Stems / enzymology
-
Plants, Genetically Modified
-
Promoter Regions, Genetic / genetics
-
Protein Transport / drug effects
-
Sucrose / pharmacology
Substances
-
Antibodies
-
Arabidopsis Proteins
-
Sucrose
-
Glucuronidase
-
Cellulase
-
Cellulose 1,4-beta-Cellobiosidase