Abstract
Adult human bone marrow stromal cells (hMSCs) grown in suspension culture gave rise to spheres of neural progenitor (NP) cells, capable of expressing both dopaminergic (DA) and GABAergic (GABA) traits. After transplantation into the Parkinsonian rat, human NPs and neurons were present at 2 weeks. Although no DA neurons appeared to survive transplantation, there were abundant GABA neurons present in the graft. By 4 weeks, however, all cells had died. Finding ways to prolong survival and promote the appropriate neurotransmitter phenotype is essential if hMSCs are to be clinically useful.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Biomarkers / metabolism
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Bone Marrow Transplantation / methods*
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Cell Death / physiology
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Cell Differentiation / drug effects
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Cell Differentiation / physiology
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Cell Line
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Cells, Cultured
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Disease Models, Animal
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Dopamine / metabolism
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Graft Survival / drug effects
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Graft Survival / physiology
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Male
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Nerve Growth Factors / pharmacology
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Nerve Regeneration / drug effects
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Nerve Regeneration / physiology
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Nerve Tissue Proteins / metabolism
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Neurons / cytology*
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Neurons / drug effects
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Neurons / metabolism
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Parkinsonian Disorders / metabolism
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Parkinsonian Disorders / physiopathology
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Parkinsonian Disorders / therapy*
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Rats
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Spheroids, Cellular / cytology
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Spheroids, Cellular / metabolism
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Spheroids, Cellular / transplantation
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Stem Cell Transplantation / methods*
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Stem Cells / cytology*
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Stem Cells / drug effects
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Stem Cells / metabolism
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Stromal Cells / cytology
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Stromal Cells / drug effects
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Stromal Cells / metabolism
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Stromal Cells / transplantation*
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Treatment Failure
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gamma-Aminobutyric Acid / metabolism
Substances
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Biomarkers
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Nerve Growth Factors
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Nerve Tissue Proteins
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gamma-Aminobutyric Acid
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Dopamine