Fluorophore-encapsulated solid-supported bilayer vesicles: a method for studying membrane permeation processes

Langmuir. 2006 Jul 18;22(15):6473-6. doi: 10.1021/la060853q.

Abstract

This letter describes a new method for studying the interaction of the membrane-lysing enzyme phospholipase A(2) (PLA(2)) with phospholipid bilayers by simultaneous measurements of enzyme binding and vesicle lysis using surface plasmon resonance (SPR) and permeabilization using surface plasmon field-enhanced fluorescence spectroscopy (SPFS). The PLA(2) inhibitor dimethyl-eicosadienoic acid was incorporated into the surface-bound vesicles and support bilayer in order to study its role in preventing PLA(2)-mediated vesicle lysis. This methodology has a generic applicability for the study of a range of membrane-disrupting agents.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fatty Acids, Unsaturated / chemistry
  • Fatty Acids, Unsaturated / pharmacology
  • Fluorescent Dyes / chemistry*
  • Lipid Bilayers / chemistry*
  • Phospholipases A / antagonists & inhibitors
  • Phospholipases A / chemistry*
  • Phospholipids / chemistry*
  • Sensitivity and Specificity
  • Spectrometry, Fluorescence / methods
  • Surface Plasmon Resonance / methods
  • Surface Properties
  • Time Factors

Substances

  • Fatty Acids, Unsaturated
  • Fluorescent Dyes
  • Lipid Bilayers
  • Phospholipids
  • 7,7-dimethyl-5,8-eicosadienoic acid
  • Phospholipases A